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plasmid vector pbluescript ii  (ATCC)


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    Structured Review

    ATCC plasmid vector pbluescript ii
    Construction scheme for the cloning plasmid <t>pBluescript</t> I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)
    Plasmid Vector Pbluescript Ii, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid vector pbluescript ii/product/ATCC
    Average 93 stars, based on 31 article reviews
    plasmid vector pbluescript ii - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system"

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    Journal: BioTechnologia

    doi: 10.5114/bta.2023.130728

    Construction scheme for the cloning plasmid pBluescript I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)
    Figure Legend Snippet: Construction scheme for the cloning plasmid pBluescript I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)

    Techniques Used: Cloning, Plasmid Preparation, Mutagenesis, Sequencing, Coagulation

    Primers for the construction of the cloning plasmid pBluescript I
    Figure Legend Snippet: Primers for the construction of the cloning plasmid pBluescript I

    Techniques Used: Cloning, Plasmid Preparation

    Construction scheme for the cloning plasmid pBluescript + Fragment 1 + Fragment 2 + Fragment 3; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of the blood coagulation factor VIII; AmpR – ampicillin resistance gene, ori – origin of replication (Vector NTI AdvanceTM10)
    Figure Legend Snippet: Construction scheme for the cloning plasmid pBluescript + Fragment 1 + Fragment 2 + Fragment 3; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of the blood coagulation factor VIII; AmpR – ampicillin resistance gene, ori – origin of replication (Vector NTI AdvanceTM10)

    Techniques Used: Cloning, Plasmid Preparation, Mutagenesis, Sequencing, Coagulation



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    Image Search Results


    Construction scheme for the cloning plasmid pBluescript I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)

    Journal: BioTechnologia

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    doi: 10.5114/bta.2023.130728

    Figure Lengend Snippet: Construction scheme for the cloning plasmid pBluescript I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)

    Article Snippet: The plasmid vector pBluescript II (GenBank Accession No. ATCC 87047) carries the ColE1 replicon and the ampicillin resistance gene (ampR).

    Techniques: Cloning, Plasmid Preparation, Mutagenesis, Sequencing, Coagulation

    Primers for the construction of the cloning plasmid pBluescript I

    Journal: BioTechnologia

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    doi: 10.5114/bta.2023.130728

    Figure Lengend Snippet: Primers for the construction of the cloning plasmid pBluescript I

    Article Snippet: The plasmid vector pBluescript II (GenBank Accession No. ATCC 87047) carries the ColE1 replicon and the ampicillin resistance gene (ampR).

    Techniques: Cloning, Plasmid Preparation

    Construction scheme for the cloning plasmid pBluescript + Fragment 1 + Fragment 2 + Fragment 3; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of the blood coagulation factor VIII; AmpR – ampicillin resistance gene, ori – origin of replication (Vector NTI AdvanceTM10)

    Journal: BioTechnologia

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    doi: 10.5114/bta.2023.130728

    Figure Lengend Snippet: Construction scheme for the cloning plasmid pBluescript + Fragment 1 + Fragment 2 + Fragment 3; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of the blood coagulation factor VIII; AmpR – ampicillin resistance gene, ori – origin of replication (Vector NTI AdvanceTM10)

    Article Snippet: The plasmid vector pBluescript II (GenBank Accession No. ATCC 87047) carries the ColE1 replicon and the ampicillin resistance gene (ampR).

    Techniques: Cloning, Plasmid Preparation, Mutagenesis, Sequencing, Coagulation

    Plasmids and oligonucleotides used.

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Identification of the Leishmania major Proteins LmjF07.0430, LmjF07.0440, and LmjF27.2440 as Components of Fatty Acid Synthase II

    doi: 10.1155/2009/950864

    Figure Lengend Snippet: Plasmids and oligonucleotides used.

    Article Snippet: Sequential electrophoresis, processing, and tethering of amplicons to an Eco RV-digested pBluescript II plasmid vector (Stratagene, La Jolla, CA) are described [ ].

    Techniques: Plasmid Preparation, Clone Assay